Redistributions Under Wnt model. Left: When Wnt is OFF, the chromatin is not accessible at enhancers of Wnt target genes, and transcription is low. CTCF binding is constitutive at a region near the promoter of the gene. Right: When Wnt is activated, the enhancer region becomes accessible, and TCF/LEF and beta-catenin bind. CTCF binds the RUW site and, with cohesin, mediates enhancer–promoter looping, which boosts target gene expression.