Ratio of FRA to DNA in different genomes
| Species | Genome size | Cell input number | DNA equivalent (μg) | FRA volume (μL) |
|---|---|---|---|---|
| Human GM12878 | 3.2 Gb | 1 × 106 | 5–6 | 1 |
| Human HEK293 | 3.2 Gb | 1 × 106 | 6–8a | 1 |
| Mongolian Gerbil | ∼2.7 Gb | Unknown | 10 | 1.5 |
| Tick A. variegatum | ∼6.0 Gb | 1 × 106 | 11–13 | 2.5 |
| Yeast S. cerevisiae (n) | 12 Mb | 2 × 108 | 4–5 | 6 |
[i] aExtracted DNA yield is likely to be more than normal diploid human cell line because of its hypotriploid nature (Lin 2014).