UL sequencing output and metrics of non-GM12878 cells and different sequencing platforms: gerbil blood, tick A. variegatum, yeast S. cerevisiae, and human HEK293 cells using either the ULK001 or the ULK001-Nemo protocol (as labeled). (A) Read length distributions of libraries run on PromethION R9.4.1 flow cells; dashed black vertical lines denote N50s. (B) Read length distribution of the HEK293 and yeast S. cerevisiae libraries run on MinION R9.4.1 flow cells. (C) Sequencing metrics of the libraries. Each metric was shown after 6 h of run (excluding the first 10 min).