METTL2A downregulates m³C RNAs. (A) Hexagonal two-dimensional heat map showing the correlation of expression changes between the two siRNAs in the direct RNA sequencing data. Statistical significance was determined by Spearman's correlation test. 2A KD: METTL2A knockdown. (B) Venn diagrams showing the number of transcripts upregulated or downregulated by each siRNA in the direct RNA sequencing data. (C) Stacked bar plots showing the percentage of m³C RNAs and other RNAs. (D) Venn diagrams showing the overlap between m³C RNAs and differentially expressed transcripts following METTL2A knockdown. (E) The top 10 biological processes enriched in the upregulated and downregulated transcripts, in red and blue, respectively.