METTL2A-mediated m³C sites are enriched in the CC motif of poly(A) RNA. (A) Schematic overview of the comparative direct RNA sequencing used to map m³C sites in poly(A) RNA. (B) Number of sites where signal alterations were detected for each parameter or method. (C) Logo plot of k-mer sequences with increased current intensity on METTL2A knockdown. (D) Flowchart illustrating the determination of m³C sites. (E) Relative distribution of m³C RNAs across different RNA classes. (F) Relative distribution of m³C sites across different RNA classes. (G) Percentage of m³C RNAs per chromosome. (H) Top 10 biological processes associated with the m³C RNAs, with mitochondrial-related processes highlighted in red.