ONT assemblies are concordant with HiFi reads Variant caller Clair3 estimated single-nucleotide variants (SNVs; A) and insertions/deletions (indels; B) between the donors’ HiFi reads and their haplotype-resolved IGH assembly from ONT data. (A) Scatter plot of HiFi read depth and variant allele frequency for SNVs from all donors. (B) Dot plot of quality score of indels called, stratified by different filtration. No filter (blue), indels within ±1 bp of homopolymers ≥5 bp (green), and indels within homopolymers or short tandem repeats of dinucleotide of seven or more repeats (orange).