Overview of DIISCO framework. (A) General workflow of the DIISCO algorithm including inputs and outputs. Cell type proportions are computed from scRNA-seq data in each time point. Expression of R–L complexes is incorporated to obtain time-resolved interactions between cell types. (B) Network diagram of the model framework. (C) Graphical model of DIISCO, including all hyperparameters. Latent variables are depicted as white circles, and observed variables are depicted as yellow-shaded circles. (D) Process for incorporating domain knowledge on R–L interactions as a prior for cell–cell interactions into DIISCO.