Bacteria strains used in this study
| Strain | Nickname | Source | Use |
|---|---|---|---|
| BW25113 ΔgspI::carb | Viability reporter strain; ampicillin-resistant reporter strain | This study | Colibactin impact on cell viability, mutation accumulation |
| BW25113 pBeloBAC11 + pks | Engineered pks+ | (Silpe et al. 2022) | Colibactin impact on cell viability, genetic screens |
| BW25113 pBeloBAC11 | Engineered pks− | (Silpe et al. 2022) | Colibactin impact on cell viability, genetic screens |
| Pooled E. coli library | Pooled E. coli library | Hirotada Mori, Nara Institute of Science and Technology, Japan | Genetic screens |
| BW25113 pBeloBAC11 + pks pRecA | Engineered pks+ with DNA damage reporter | This study | Self-inflicted damage |
| BW25113 pBeloBAC11 pRecA | Engineered pks− with DNA damage reporter | This study | Self-inflicted damage |
| Nissle 1917 pRecA | Nissle 1917 pks+ DNA damage reporter | This study | Self-inflicted damage |
| Nissle 1917 ΔclbN::chl pRecA | Nissle 1917 pks− DNA damage reporter | This study | Self-inflicted damage |
| Nissle 1917 ΔclbS::chl pRecA | Nissle 1917 pks+ clbS knockout DNA damage reporter | This study | Self-inflicted damage |