Simulated nanopore signal data with tunable parameters. Examples show experimental and simulated data from a single DNA sequence, showcasing some of the different simulation parameters available in Squigulator. (A) Experimental nanopore raw signal data (top track) versus simulated data with no noise in the amplitude or time domains (‐‐ideal option; middle track) versus the -x dna-r9-prom preset configuration (bottom track), which is currently the default option in Squigulator. This configuration emulates standard sequencing of genomic DNA on an ONT PromethION R9.4.1 flow cell, matching the experimental data above. (B) Simulated data with varying DNA translocation speeds (‐‐bps option). (C) Simulated data with varying data acquisition rates (‐‐sample-rate option). (D) Simulated data with varying data digitization levels (‐‐digitisation option).