hAT-mediated male-specific transcriptional regulation of the gsdfY promoter by Dmrt1 and Wt1(-KTS). (A–C) In vitro quantification of proximal gsdfX and gsdfY promoter activities (luciferase reporters), after Dmrt1 and Wt1(-KTS) transient transfection in OLF fibroblast (A), SG3 spermatogonia (B), and MES1 embryonic stem (C) cell lines from medaka (Oryzias latipes). For every construct, transfection was repeated six times. Error bars represent the standard deviation of the means. See the Methods section for detailed information about the luciferase constructs. See also Supplemental Data S1 for raw data.