The effect of controlling for GC content bias in Nubeam. (A) GC content (x-axis) versus bin coverage (y-axis) to demonstrate patterns of GC bias for three simulation schemes, with LOESS curve marked in blue. Recall that $r_b=\left({\overline{d}}_b-{\overline{d}}_0\right)/{\overline{d}}_0$ and $r_c=\left({\overline{d}}_c-{\overline{d}}_0\right)/{\overline{d}}_0$ measure relative differences before and after controlling for GC bias, respectively. (B) r_c = 2.4% after controlling for GC bias versus r_b = 50.7% before controlling for GC bias for simulation scheme 1. (C) r_c = 7.1% after controlling for GC bias versus r_b = 78.2% before controlling for GC bias for simulation scheme 2. (D) r_c = 4.9% after controlling for GC bias versus r_b = 38.6% before controlling for GC bias for simulation scheme 3.