Flow chart of generation of 5518 UAS-cDNA/ORF constructs within 3 mo, in which 6515 DGRC cDNA/ORF plasmids covering 6515 Drosophila genes conserved in humans were subjected to CRISPRmass reactions. At least one colony grew on LB agar plates with appropriate antibiotics for 5987 reactions after transformation. Only one colony from each plate was randomly picked up for subsequent restriction analysis. UAS module-specific fragments and UAS module-vector assembly-specific fragments were observed in 5903 constructs, demonstrating that UAS modules were inserted into 98.6% of the 5987 constructs successfully, resulting in 5903 UAS-cDNA/ORF constructs. After a series of analyses of 5903 UAS-cDNA/ORF constructs were conducted, 5518 UAS-cDNA/ORF constructs covering 5518 Drosophila genes conserved in humans were retained as a resource.