Prenatal and adult human cortices show distinct patterns of RNA localization across the nuclear membrane. (A) MA plots of prenatal and adult gene expression differences across fraction. (B) Log₂ fold change of gene expression across fraction in adult poly(A)+ samples (x-axis) or prenatal poly(A)+ samples (y-axis). (C) Log₂ fold change between fraction and age for six developmentally regulated gene sets: Colors indicate developmental expression direction, and columns indicate the fraction in which the developmental expression difference is detected. The y-axis shows the log₂ fold change between fractions stratified by the age in which the fraction differences are measured, and the x-axis shows the log₂ fold change between ages as measured in cytoplasm. Although these developmentally regulated gene sets are similar across prenatal fractions, down-regulated genes in adult are more sequestered in the nuclear compartment.