ACMG classification of NRAS G12S. (Top) ProteinPaint display of somatic NRAS variants across 28 cancer subtypes, showing hotspot at G12. Arrow indicates position of germline G12S variant. (Bottom) Detail of PeCanPIE ACMG classification interface for G12S variant. Automated classification detected a pathogenic ClinVar variant at the same amino acid position as well as a hotspot in COSMIC data.