Retrotransposition in the early embryo. (A) Schematic of insertion #5 in the antisense orientation within the second intron of the gene Ano4 on Chr 10, and the donor element of insertion #5 on Chr 1. Features are annotated as in Figure 2A. (B) Genotyping panel for insertion #5. SRE (maternal) and SRF (paternal) tissues are indicated. (Somatic) Mix of liver, heart, and brain genomic DNA; (R.O., L.O.) right ovary, left ovary; (R.T., L.T.) right testicle, left testicle. (C) Top: Control assay demonstrating the ability of the qPCR assay to detect mosaicism for insertion #5. Data are reported as the mean and standard deviation of four technical replicates per reaction. Bottom: Prevalence of insertion #5 among the tissues of maternal mouse SRE. Liver genomic DNA from mouse SREF18, a heterozygote for insertion #5, is set to 100%. Mouse SREF15, which lacks insertion #5, is included as a negative control. Data are reported as the mean and standard deviation of three independent qPCR experiments, each comprising four technical replicates per reaction.