Long-range epigenetically activated (LREA) domains occur at differential interactions in cancer cells. The majority of LREA regions overlap differential interactions in cancer cells. Anchor points of differential interactions are visualized in Rondo simultaneously with ChIP-seq (H3K27ac, H3K4me3, and H3K27me3), RefSeq genes, and RNA-seq data. (A) In normal PrEC cells, there are no interactions evident across the LREA region within Chr12:81,000,000–82,200,000, which is consistent with the low levels of H3K4me3 (dark green), absence of H3K27ac (light green), and gene inactivity (RNA-seq, dark cyan). (B) In LNCaP prostate cancer cells, three new, distinct interactions (orange) are observed. Most of the genes are highly expressed (RNA-seq) and active marks (H3K4me3, dark green; H3K27ac, light green) are acquired. This region is devoid of repressive H3K27me3 marks (pink). (C) A combined view shows normal and cancer epigenomes, expression and interaction data simultaneously. Only interactions present in LNCaP (cancer; orange) are evident, with no shared interactions. The circular tracks depict gene expression (RNA-seq) and histone marks (H3K4me3, dark green; H3K27ac, light green; H3K27me3, pink).