Composition and function of select DNA replication and chromatin regulation complexes
| Complex name | Component names | Function |
|---|---|---|
| Origin recognition complex | Orc1, Orc2, Orc3, Orc4, Orc5, Orc6 | Binds to origins of DNA replication |
| Minichromosome maintenance complex | Mcm2, Mcm3, dpa, Mcm5, Mcm6, Mcm7, Mcm10 | DNA helicase unwinds dsDNA |
| Replication factors | Cdc45, dup, geminin, cdc6 | Factors regulating initiation of the replisome |
| Replication Protein A | RPA1, RPA2, RPA3 | Binds to single stranded DNA |
| DNA polymerase epsilon | Chrac-14, PolE1, PolE2, PolE4 | Synthesizes leading strand DNA |
| DNA polymerase alpha | PolA1, PolA2, Prim1, Prim2 | Synthesizes the RNA primer that initiates DNA synthesis |
| DNA polymerase delta | PolD1, PolD2, PolD3 | Synthesizes lagging strand DNA |
| Other DNA polymerases | PolH, PolZ1, PolZ2, PolI, PolG1, PolG2, PolQ | Varied polymerases for DNA repair and mitochondrial DNA synthesis |
| Replication factor C/Egl1 complex | CG8142, elg1, RfC3, RfC4, RfC38 | Regulates chromatin association of PCNA |
| DNA ligases | DNAlig1, DNAlig3, DNAlig4 | Joins DNA strands |
| DNA Helicases | Dna2, RecQ4, RecQ5, Pif1 | DNA helicases functioning in DNA repair |
| DNA N-glycosylases | Tdg, Ogg1, Nthl1 | Enzymes that remove damaged bases from DNA |
| DNA polymerase Rev1 | Rev1 | DNA polymerase involved in translesion synthesis |
| DNA repair | Brca2 | Involved in DNA repair |
| Chromatin assembly factor 1 | Caf1-180, Caf1-105, Caf1-55 | Assembles nucleosomes on newly synthesized DNA |
| Chromatin Accessibility Complex | Acf, Chrac-14, Chrac-16, Iswi | Regulates chromatin accessibility through nucleosome sliding |
| Histone variants | His4r, Cid, His2Av | Histone variants |
| Histone chaperones | Hira, Nap1 | Histone chaperones |
| Nucleosome remodeling factor | Nurf-38, Caf1-55, E(bx), Iswi | Nucleosome sliding complex regulates accessibility for transcription |
| Remodeling and spacing factor complex | CG8677, Iswi | Remodeling and spacing factor complex |
| SNF2-like chromatin remodelers and NuRD | Mi-2, dom, chd3 | Disrupts DNA–histone interactions |
| FACT | dre4, Ssrp | Interacts with H2A-H2B dimers and facilitates nucleosome formation |
[i] Components in bold are differentially enriched in GSC-like cells.