Evaluation of the presented targeted demethylation approach on high-confident m⁶A targets with >0.5 methylation stoichiometries. SELECT-qPCR data and normalized m⁶A abundance are shown for LAMTOR2 (NM_014017.4 m⁶A:332; A), CDK2 (NM_001798.5 m⁶A:1158; B), and NAGLU (NM_000263.4 m⁶A:2002; C) at 48 h posttransfection with Dem6A-Vec expressing the target gRNAs.