Distribution of poly(A)⁺ RNA 3′-end tags (PATs) upon inactivation of the nuclear exosome. (A) Strategy for mapping 3′-ends of RNAs targeted by the nuclear exosome. (B) Global distribution of PATs in WT and rrp6Δ. PATs mapping to the indicated regions were tallied and grouped by the indicated genomic annotations. (C) Global distribution of PATs upon nuclear depletion of Rrp6p or codepletion of Rrp6p and Dis3p by the anchor away system. (D) Distribution of PATs clusters up-regulated in RRP6-FRB and RRP6/DIS3-FRB strains relative to WT (see Methods).