X chromosome demasculinization was observed for incRNAs as well as coding transfrags, based on comparison of whole body of males versus females (A), testes versus ovaries (B), and accessory glands versus ovaries (C). Comparison of the gut and thorax of males versus females is shown in D and E, respectively. Odds ratios >1 indicate enrichment on autosomes, and <1 indicates enrichment on X chromosome. Significant deviations are indicated: (***) P < 0.001; (**) P < 0.01, Fisher's exact test. Blue and red bars represent male-biased and female-biased transfrags, respectively.