In situ hybridization screen and qPCR tests validate CNC transcriptome data set. In situ hybridization confirms enrichment of migrating CNC genes. These include well-known neural crest markers such as SOX10, ETS1, and SNAI2 (A–C) as well as novel genes previously not known to be expressed in the migrating CNC (D–T). (U) qPCR tests corroborate enrichment found in the RNA-seq data. Vertical error bars on data points represent the standard error of mean obtained from replicates.