Sequencing of B-cell receptor repertoires. (A) Representation of the genomic rearrangement process during V-D-J recombination to generate the heavy-chain B-cell receptor. B-cell receptor amplification was performed by reverse transcription on total RNA by single J region primer, and subsequent multiplex PCR amplification. (B) The percentage of reads corresponding to the highest expressed B-cell receptor sequence for each sample, separated into sample type: healthy individuals, chronic lymphocytic leukemia patients (CLL), and human lymphoblastoid cell lines (LCL). Two-sided t-tests were performed between the sample subsets, with the P-values indicated above. (C) Percentage of sequences shared between runs for technical repeats for (1) the RT-PCR and resequencing (RT-PCR repeats, green bars), and (2) the 454 sequencing from the same RT-PCR product (sequencing repeats, purple bars). For each sample, two repeats were performed and the percentage of reads shared between the repeats is shown (each repeat is compared with the other, so two bars are shown per sample).