Validation and functional characterization of enriched families of genes from genome-scale screen. (A–F) Members of enriched families of genes were validated by Western blotting. Drosophila S6_S2R⁺ cells were incubated with dsRNAs targeting the indicated genes from groups identified in Figure 2E, and lysates were analyzed by Western blotting. See also Supplemental Table S1 for results and analysis related to the genome screen.