Clusters of interacting proteins implicated in Ty transposition. ORFs encoding proteins that affected transposition were entered into GOnet, and interacting protein pairs as determined by all methods (synthetic lethal, two hybrid, and affinity purification) were downloaded for display in Cytoscape. To illustrate clusters of interacting proteins in this figure, only relatively small subsets of genes were queried for interaction networks. (A) Eleven ESCRT-related protein genes (SNF8, VPS36, VPS28, VPS25, VPS20, SNF7, VPS24, VPS4, BRO1, RIM13 and RIM20); ESCRT complex genes are encircled. (B) Nuclear transport genes; Nup84 subcomplex component genes are encircled (Table 1). (C) DNA maintenance genes (Table 1). (D) ELG1 and RAD24, homologs of clamp loader subunit Rfc1. Red indicates those identified in the current Ty3 screen; blue, those identified in the Griffith et al. (2003) Ty1 screen; yellow, those identified in the Scholes et al. (2001) Ty1 screen; purple, those identified in the Griffith et al. screen and the Ty3 screen; and orange, those identified in the Scholes et al. screen and the Ty3 screen.