The formation of nucleosome arrays on a plasmid containing a 1899-bp FRA3B distal breakpoint sequence. A diagram of the plasmid with a 1899-bp FRA3B distal breakpoint sequence inserted at the SmaI site of the polylinker region (white bar) of the pGEM3zf(+) vector is shown. The single arrow indicates the direction of the FHIT gene. The position of the distal breakpoint cluster of the FRA3B site is indicated by a blast. The position of the high-flexibility DNA sequence is indicated by a hatched bar. (E) EcoRI; (A) AflIII. RSF-assembled plasmids (lanes 1–4, the plasmid containing the FRA3B sequence; lanes 5–8, the parental plasmid) were partially digested by two different concentrations of MNase. The MNase-treated DNA was purified, and half of the DNA was used directly in lanes 1, 2, 5, and 6. The other half of the DNA samples was further digested by HindIII (lanes 3,4,7,8), and the MNase digestion patterns were analyzed by Southern blots. The 26-nt hybridization probe is located between the HindIII and SmaI sites. The multiple arrows to the left indicate the nucleosome ladder.