(A) Fluorescence electropherogram for a 5382insC mutant. Peaks are clear before the mutation site and unintelligible beyond the mutation site (after “C” at position 118). (B) Mass spectrum showing the sequencing data for a 5382insC mutant. The appearance of a succession of clear double peaks at and after the third position confirms the presence of the insertion of a “C”. The wild-type DNA sequence is 5′-... CCAGGA-... 3′, whereas the mutant DNA sequence is 5′ -... CCCAGG... -3′.