Estimates of Constraint in Runs of Nucleotides Close to the 5′- or 3′-Ends of Introns, Calculated Using the Two Lineage Method, Applied to Sequences from D. melanogaster and D. simulans
Substitutions | |||||
|---|---|---|---|---|---|
| Intron end | Base pairs | Observed (SEM) | Expected (SEM) | Constraint (SEM) | |
| 5′ | 1–2 | 0 (0.0) | 0.294 (0.021) | 1.00 (0.00) | |
| 3–4 | 0.145 (0.037) | 0.277 (0.021) | 0.47 (0.13) | ||
| 5–6 | 0.055 (0.024) | 0.295 (0.022) | 0.82 (0.077) | ||
| 3′ | 1–2 | 0 (0.0) | 0.292 (0.021) | 1.00 (0.00) | |
| 3–16 | 2.248 (0.202) | 1.912 (0.142) | –0.178 (0.098) | ||