Table 1.

Deletion mutants for enhanced hydrogen production in E. coli

Secretion per hour
(mmol/gDW)
No. of knockouts
ID
Reaction
Enzyme
Growth rate (L/h)
CO2
H2
2 A 1. 2PG ↔ H2O + PEP Enolase 0.227 32.7 22.8
2. G6P + NADP ↔ 6PGL + H+ + NADPH Glucose-6-phosphate
3 B 1. ADP + 4 H+ + PI ↔ ATP + 3 H+ + H2O ATP synthase 0.174 40.9 29.5
2. AC + ATP ↔ ACTP + ADP Acetate kinase
3. AKG + CoA + NAD → CO2 + NADH + SUCCoA
2-Oxogluterate dehydrogenase

[i] A basis glucose uptake rate of 10 mmol/gDW per hour is assumed.