Examples of Acceptable Clones Found
Target RefSeq ID | Gene name | PCR band size | Contig size | Comments |
|---|---|---|---|---|
| NM_000871 | HTR6 | ∼1400 bp | 1401 bp | Matches ORF perfectly.a |
| NM_000910 | NPY2R | ∼1200 bp | 1203 bp | T627Cb I->I (silent),c T978C I->I (silent) |
| NM_001971 | ELA1 | ∼800 bp | 822 bp | T541C Y->Y (silent), C680G L->V (Genomic validationd - BLAT) |
| NM_003308 | TSPY | ∼1000 bp | 978 bp | A161G V->V (silent), CCCG301 GGGC PR->RA (Genomic validation - BLAT). |
| G458C H->Q validated by dbEST. | ||||
| NM_004347 | CASP5 | ∼1300 bp | 1320 bp | G975C V->L validated by Ensembl dbSNP as an SNP. |
a Based on RefSeq sequence.
b Base change in clone insert vs. RefSeq.
c Effect of sequence change on protein coding.
d Validation means confirming the change as known polymorphism, based on current databases.