GFP (A,C) and β-galactosidase (B,D) expression, in the head region of transgenic adult C. elegans, driven by F44B9.2 (A,B) and B0464.4 (C,D) promoter fragments. The gfp reporter gene fusions were generated using MultiSite Gateway while the lacZ reporter gene fusions had been generated previously by conventional cloning. Expression is in body wall muscle cells for F44B9.2 and in nerve cells for B0464.4. Micrographs of C. elegans strains UL1110 (A), UL484 (B), UL1111 (C), and UL42 (D) were captured at 400× magnification.