Real-time PCR quantification of mRNA expression levels of candidate genes for plasma HDL concentrations in B6 and NZB mice. Total RNA was extracted from the livers of female mice-fed chow and from mice fed a high-fat diet for 4 weeks. It was then transcribed into cDNA. mRNA expression levels for each candidate gene was quantified with real time PCR by using fluorescent SYBR. Results were normalized to Gapd and expressed as mRNA copies of candidates per 1000 copies of Gapd. (^*P < 0.05, and ^**P < 0.01, compared with B6 mice on the same diet).