Table 3.

Comparison of Putataive and Predicted Virulence Factors in Four Strains of GAS Genomes

Strains
Gene description
SSI-1 (sps)a
SF370 (spy)a
MGAS315 (spyM3_)a
MGAS8232 (spyM18_)a
Locationb
Proteinase
C3 degrading proteinase 0269 1851 1598 1914 Ch
Exfoliative toxin-like protein 1220 0918 0632 0975 Ch
IdeS/Sib38 1270 0861 0583 0921 Ch
Streptokinase A 1700 1979 1698 2042 Ch
C5a peptidase 1724 2010 1726 2074 Ch
SpeB/Cysteine proteinase 1739 2039 1742 2099 Ch
Serine proteinase 1860 2216 1864 2256 Ch
Adhesin
GRAB 0828 1357 1032 1369 Ch
SclA (1704)c 1983 1702/1703 — Ch
SclB 0939 1054 0738 1029 Ch
M protein 1725 2018 1727 2076 Ch
Fibronectin-binding protein F2-like 0106 — 0104 0132 Ch
Fibronectin-binding protein (Fba)-like — 2009 — —
Internalin-like protein 0825 1361 1035 1373 Ch
Laminin-binding protein 1723 2007 1725 2073 Ch
Hemolysin
SLO 0132 0167 0130 0165 Ch
SLS 1366 0746 0488 0807 Ch
CAMP 1104 1273 0905 1221 Ch
Hemolysin (hylA1) 0709 1497 1153 1515 Ch
Hemolysin homolog (hylX) 1583 0378 0276 0432 Ch
Hemolysin homolog (hyl III) 1016 1159 0815 1119 Ch
Capsule synthesis and degradation
Hyalunonate synthase (hasA) 1847 2200 1851 2236 Ch
Hyaluronidase 0567 1600 1294 1606 Ch
Hyaluronidase 0447 — 1418 — Ph
Hyaluronidase 1127 — 1418 — Ph
Hyaluronidase 0763 — 1108 — Ph
Hyaluronidase 0927 — 1101 — Ph
Hyaluronidase (hylP1) 0648 0701 1214 — Ph
Hyaluronidase (hylP2) — 0997 — — Ph
Hyaluronidase (hylP3) — 1445 — — Ph
Hyaluronidase (hyl) — — — 0385 Ph
Hyaluronidase (hyl) — — — 0770 Ph
Hyaluronidase (hyl) — — — 1254 Ph
Hyaluronidase (hyl) — — — 1455 Ph
DNase
Mitogenic factor (mf) 1743 2043 1745 2104 Ch
Mitogenic factor-like 0770 — 1095 — Ph
MF2 (mf2) — 0712 — 0779 Ch
MF3 (mf3) — 1436 — — Ph
Sda 0455 — 1409 1746 Ph
Superantigen
SpeA 0560 — 1301 0393 Ph
SpeC — 0861 — 0778 Ph
SpeG 0161 0212 0155 0201 Ch
SpeH — 1008 — — Ph
Spel — 1007 — — Ph
SpeJ — — — 0919 Ch
SpeK 0657 — 1205 — Ph
SpeLd 0657 — 1205 1238 Ph
SpeM — — 1239 Ph
SmeZ — 1998 — 2064 Ch
SSA 1119 — 0920 — Ph
Immunoreactive antigen
Immunogenic secreted protein 0305 1801 1562 1870 Ch
Myosin crossreactive antigen 1525 0470 0332 0512 Ch
Immunogenic secreted protein 1728 2025 1731 2082 Ch
Regulators
SpeB protease transcription regulator (rgg) 1742 2042 1744 2103 Ch
RopA (trigger factor) 0232 1896 1634 1961 Ch
M protein transacting positive regulator 1726 2019 1728 2077 Ch
CsrR/S (CovR/S)e 1614/1615 0337 0244/0245 328/0329 Ch
RofA 0163 0216 0157 0205 Ch
Bacteriocin
Salivaricin precursor 1650 1915 1652 1983 Ch
SalK homolog 1646 (1910)c 1646 1979 Ch
Others
Sic — 2016 — — Ch

a Numbers in this table indicate ORF numbers annotated in GenBank Database (SF370:AE004092, MGAS8232: AR009949 and MGAS315: AE014074).

b “Location” indicates the genes located in backbone chromosome (Ch) or in the phage sequences (Ph).

c ORF number in parentheses indicates a disrupted gene by frameshift mutation.

d Nucleotide sequence of SpeL gene in GenBank (NP_438166) is different from that annotated in MGAS8232 (AAL97848.1)

e CsrR was not annotated in GenBank (AE004092), however, nucleotide sequence for CsrR is found in SF370 genome.