Primer design strategy for amplification of the 3′ ends of the annotated genes identified on the chromosome 2. Starting at the predicted stop codon of each annotated gene, we selected a region 1000 bp upstream and 150 bp, or the length of the annotated 3′ untranslated region if available and extracted it from the genomic sequence. Primer 3.0.9 from the Whitehead Institute was used to design primers spanning >5/6 the length of the selected region. Amplification success from genomic DNA was 94.2% using this approach.