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Table 1.

List of S. cerevisiae smORFs for Which a Transcript was Detected

smorf	Original name	Chromososme	Coordinates	Size (aa)	CAI	RT-PCR product (bp)	SGD annotation	Notes
3	SR13	chr1	124491–124306	62	0.243	39
13		chr2	18437–18177	87	0.181	99
18^a	smORF2	chr2	89973–90221	82	0.154	205	YBL071W-A
19^c	SR12	chr2	91789–92025	79	0.241	200	YBL069W	embedded ORF
24	SR11	chr2	209370–209609	79	0.102	142
44	SR10	chr2	382978–382817	54	0.134	93
46		chr2	391575–391309	89	0.091	72
53	SR9	chr2	614131–613982	50	0.125	115
54	SR8	chr2	622941–623105	55	0.082	113
57^a ^b	SR7	chr2	684935–685219	94	0.105	170	YBR233W-A
68		chr3	24325–24032	98	0.149	99	YCL057C-A	removed by S GD
82^a	SR6	chr3	113722–114018	98	0.102	247	YCL001W-B
93	SR5	chr3	316181–315990	63	0.121	161
98	SR4	chr4	229171–229431	87	0.175	38
101	SR3	chr4	410052–409804	83	0.151	68
104		chr4	410052–409804	83	0.108	77
109^a ^b		chr4	603805–603587	73	0.119	31	YDR079C-A
118	SR2	chr4	794716–794567	50	0.164	124/98^e
121	SR1	chr4	829143–829346	67	0.160	155
122		chr4	830646–830389	86	0.149	31
123		chr4	848063–848215	51	0.067	64
127		chr4	955124–955324	67	0.066	99
139^a	smORF8	chr4	1233506–1233267	79	0.161	227	YDR379C-A
144		chr4	1283788–1284033	82	0.153	34
154		chr9	96725–96522	78	0.135	67
167		chr5	59549–59740	77	0.099	41
171		chr5	117380–117183	66	0.216	87
189		chr5	318642–318806	86	0.065	97
201		chr6	48734–48925	64	0.173	63
217		chr7	93078–93308	77	0.078	99
226		chr7	418882–418700	61	0.097	40
247		chr7	733407–733622	72	0.118	31
250		chr7	974773–974573	67	0.091	92
274		chr8	280232–280393	54	0.095	79
283		chr8	453556–453705	50	0.084	75
286		chr8	467058–466954	55	0.102	74
288		chr8	466928–467110	61	0.079	62
298		chr10	181408–181250	53	0.158	99
301^a ^b		chr10	316419–316676	86	0.106	99	YJL062W-A
303^a		chr10	411124–410927	66	0.183	94	YJL012C-A
313		chr10	637622–637861	80	0.081	99
318		chr10	717273–717323	74	0.132	42
324		chr11	98609–98397	71	0.186	25
337		chr11	447674–447432	81	0.156	27
352		chr12	136344–136520	59	0.197	85
363		chr12	455434–455637	68	0.105	34
382		chr12	455402–455566	55	0.078	99
392		chr12	454697–455071	125	0.170	98
398		chr12	454393–454557	55	0.059	99
421		chr12	462672–462523	50	0.045	99
439		chr12	452439–452624	62	0.086	99
483		chr12	490407–490595	63	0.131	65
494^d	SR15	chr12	468959–468831	44	0.052	100
			472611–472483
			482191–482063
			485843–485715
499		chr12	582236–582006	77	0.140	37
505		chr12	708338–708168	57	0.105	45
509	SR17	chr12	760642–760355	96	0.102	190/82^e
511	SR16	chr12	815810–815983	58	0.105	125/61^e
514^a	SR18	chr12	853460–853717	85	0.282	230	YLR363W-A
519		chr12	950468–950262	69	0.135	39
526		chr13	167781–167623	53	0.129	99
530	SR25	chr13	337312–337602	97	0.082	160/58^e
532		chr13	550910–551134	75	0.121	94
540		chr13	733267–733455	63	0.158	64
543		chr13	804455–804691	79	0.101	80
544		chr13	887925–887731	65	0.094	59
556^a	SR22	chr14	330326–330544	73	0.130	170/37^e	YNL162W-A
561	SR21	chr14	381388–381242	48	0.102	90
564		chr15	4130–4312	61	0.121	55
570	smORF31	chr14	586816–386598	72	0.124	216
577		chr15	4130–4312	61	0.153	75
580		chr15	9179–9352	58	0.222	77
590		chr15	355651–355857	78	0.135	29
591		chr15	371684–371956	63	0.139	99
598		chr15	461792–462040	59	0.054	99
601		chr15	467391–467624	70	0.103	67
625		chr15	907927–907718	70	0.085	97
626		chr15	939340–939549	70	0.111	67
631		chr15	1045189–1045344	52	0.117	78
632		chr15	1058416–1058583	56	0.154	73
640		chr16	75702–75989	96	0.158	99
643		chr16	188512–188306	69	0.133	84
655		chr16	480177–480368	64	0.151	30
667		chr16	744172–744384	71	0.087	81
672		chr16	883234–883452	73	0.129	95

[i] This table indicates the chromosomal location based on SGD nucleotide coordinates. smORFs marked with an (^a) correspond to those identified by Blandin et al. (2000). smORFs marked with a (^b) sign correspond to those identified by Cliften et al. (2001). smorf19 (^c) was originally located downstream of YBL069W (AST1), which was recently extended (Saccharomyces Genome Database, 2001.http://www.genome-www.standford.edu/Saccharomyces). smorf494 (^d) is found four times in chromosome 12, downstream of theASP3 gene in a 3.6-kb repeat (Johnston et al. 1997). For smORFs marked with (^e), RT-PCR was carried with two sets of primers, one set shown in Fig. 2D and a second in Fig. 2E.