Reconstruction of the metabolic network of S. cerevisiae. Based on the available information from the genome annotation, biochemical pathway databases, biochemistry textbooks, and recent publications, a genome-scale metabolic network of S. cerevisiae was designed. Additional physiological constraints were considered and modeled, such as growth and nongrowth-dependent ATP requirements. Compartmentation was included, and cofactor requirements of all model reactions were inspected carefully, thereby, reactions that created a net transhydrogenic effect were additionally constrained. Regulatory information was not included. The picture of the pathway map was taken from the KEGG database (www.genome.ad.jp).