Table 6.

Common Effects of Novobiocin and Quinolone Antibiotic Treatment for Both Escherichia coli andHaemophilus influenzae

InEscherichia coli MG1655 InHaemophilus influenzaeKW20 %ident Drug effect
gene description gene description
b2255 Putative transformylase hi0623 Methionyl-tRNA formyltransferase28↑
fmt 10-Formyltetrahydrofolate:L-methionyl-tRNA(fMet)  N-formyltransferase hi0623 Methionyl-tRNA formyltransferase65↑
b2392 Putative transport system permease hi1728 Conserved hypothetical protein25↓
gyrA DNA gyrase, subunit A, type II topoisomerase hi1264 DNA gyrase, subunit A71↑
gyrB DNA gyrase subunit B, type II topoisomerase,  ATPase activity hi0567 DNA gyrase, subunit B75↑
nusB Transcription termination; L factor hi1304 N utilization substance protein B55↑
topA DNA topoisomerase type I, omega protein hi1365 DNA topoisomerase I72↓
ygjU Putative transport protein hi1545 Sodium dicarboxylate symporter protein62↓
dctA Uptake of C4-dicarboxylic acids hi1154 Proton glutamate symporter protein, putative25↓
gmhA Phosphoheptose isomerase hi1181 Phosphoheptose isomerase74↓
yraO Orf, hypothetical protein hi1181 Phosphoheptose isomerase45↓

[i] H. influenzae genes that were changed greater than two-fold in both novobiocin and ciprofloxacin treatments (Gmuender et al. 2001) were compared against E. coli genes with similar changes in both novobiocin and pefloxacin. Sequence homology scores (%Ident) were obtained from the Comprehensive Microbial Resource at TIGR (Peterson et al. 2001). P values for all gene pairs were better than 10−8.