Substitution Rate Comparison of Orphan and Nonorphan cDNAs
cDNA | Substitution Rate | Orphans | Nonorphans | Orphan/nonorphan ratio | P value |
|---|---|---|---|---|---|
| All | dS | 0.335 ± 0.0130 (n = 100) | 0.277 ± 0.0060 (n = 559) | 1.2 | 1.2 × 10−4 |
| dN | 0.062 ± 0.0077 (n = 100) | 0.020 ± 0.0014 (n = 559) | 3.1 | 8.5 × 10−12 | |
| dN/dS | 0.171 ± 0.0157 (n = 100) | 0.068 ± 0.0043 (n = 559) | 2.5 | 7.8 × 10−13 | |
| Embryo | dS | 0.323 ± 0.0240 (n = 31) | 0.265 ± 0.0078 (n = 350) | 1.2 | 0.037 |
| dN | 0.069 ± 0.0189 (n = 31) | 0.016 ± 0.0013 (n = 350) | 4.3 | 5.1 × 10−4 | |
| dN/dS | 0.182 ± 0.0345 (n = 31) | 0.060 ± 0.0052 (n = 350) | 3.0 | 1.7 × 10−4 | |
| Adult | dS | 0.344 ± 0.0157 (n = 70) | 0.266 ± 0.0079 (n = 286) | 1.3 | 1.5 × 10−5 |
| dN | 0.063 ± 0.0082 (n = 70) | 0.022 ± 0.0022 (n = 286) | 2.9 | 1.3 × 10−9 | |
| dN/dS | 0.172 ± 0.0177 (n = 70) | 0.073 ± 0.0086 (n = 286) | 2.4 | 6.7 × 10−12 |
[i] Mean and standard errors of the mean are given. Significance of differences were tested using Student's t.