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Table 5.

NORFs Identified by MudPIT Proteomic Analyses

NORF ID	Proposed ORF designation	Chromosomal location	Size (AA)	CAI[i]	Peptide identified	Transcripts per cell[ii]
NAL010C	YAL063C-A	Chr I: 22400-22688	96	0.17	R.YRNKEKGKFISLCK.N	1.8
NBR028W[iii]	YBR126C-A	Chr II: 490808-491014	68	0.16	R.LHQLDGIPHA.-[v] [vi]	N.D.
NCR024W	YCR095W-A	Chr III: 289632-289790	52	0.08	H.TKVNKKSSMHAFLCKIYK.R	0.7
NDR129W	YDR320W-B	Chr IV: 1108476-1108613	45	0.13	L.NSLLFLPICFCLLQLKATCAVR.V	2.0
NDR156C	YDR379C-A	Chr IV: 1233506-1233267	79	0.16	K. DFTTIEHLLRVGNK.K	1.4
					K.ENQVNFVNYIHEEFGK.Y
NGR097C	YGR169C-C	Chr VII: 836660-836382	92	0.13	K.ERDALLTAEEEQLGK.G	1.0
					K.ERDALLTAEEEQLGKGGK.G
					K.QRAQMEQLEAEEASK.W
					X.QRAQMEQLEAEEASKWEQGSRK.E
NHL007C	YHL048C-A	Chr VIII: 5796-5662	44	0.08	G.RARMGGLIVKHRFN.H	1.2
NHR007W	YHR032W-A	Chr VIII: 175186-175365	59	0.06	G.NFKGFAMWHATGKH.H	0.7
NIL001W	YIL002W-A	Chr IX: 350298-350507	69	0.17	K.DILDVLNLLK.G	1.5
NIR003C	YIR018C-A	Chr IX: 385698-385561	45	0.10	K.RYLEIMSTASAQ.F	1.0
NIR008W	YIR021W-A	Chr IX: 398511-398723	70	0.11	K.SDFKKHSKE.I	0.7
NLR127C	YLR361C-A	Chr XII: 849678-849382	98	0.12	R.TGGHRPQISDEEVSK.R	0.8
NMR066W	YMR247W-A	Chr XIII: 769282-769425	47	0.10	S.AKLLSGIMALLFNGKSLLRP.I	0.5
NNL014W	YNL042W-B	Chr XIV: 547109-547366	85	0.11	V.RVATYICQKNESR.F	0.5
NNL029W	YN067W-B	Chr XIV: 499414-499554	46	0.11	L.MWCTGVVSKTALLTGNFFFS.S	0.4
NNL042C	YNL146C-A	Chr XIV: 351577-351383	64	0.13	S.AYYVSQVLRICKEMPYR.D	0.2
NNL058W	YNL277W-A	Chr XIV: 116677-116865	62	0.05	M.CHILPPLR.S	−0.2
NOL015W[iv]	YOL077W-A	Chr XV: 185437-185643	68	0.20	L.GLLGLLVVPNPFK.S	2.2
NOL020W	YOL097W-A	Chr XV: 136219-136404	61	0.12	Q.SMICSSEHENLTCK.Y	0.2
NOL049W	YOL155W-A	Chr XV: 27083-27217	44	0.08	G.SFNKCVTGYSCRMAIHYY.V	0.0
NOR002C	YOR034C-A	Chr XV: 397667-397425	80	0.13	R.IWVREKGRKCSFFFF.S	0.8
NPL013C	YPL119C-A	Chr XVI: 324286-324023	87	0.11	R.NIFEIGGLLLQ.S	0.5

[i] CAI values were calculated according to Sharp and Li (1987).

[ii] The transcriptional expression level of each NORF in approximate copies of transcripts per cell in a log phase cell.

[iii] Probes to NBR028W were not selected for inclusion on the S98 array.

[iv] YOL077W-A was discovered by the biochemical purification of the F₁F₀-ATP synthase complex (Arnold et al. 1998).

[v] The ‘-’ indicates the C-terminus of the protein.

[vi] The ‘.’ after an amino acid indicates the cleavage sites of the peptide. The sequence between the periods in each cell indicates the actual peptide identified by tandem mass spectrometry. (ND) there was no detectable expression in a log phase culture.

[vii] MudPIT, multidimensional protein identification technology.