Table 2.

Minimal Set of Clones Covering the T. pallidum Chromosome

Name Start coordinate Stop coordinate No. of complete ORFs Length of insert (bp) Complete ORFs encoded No. of ORFs amplified[i]
DSTP001158990[ii] 934065565585TP0084-TP013740
DSTP0035432534546298188625TP0427-TP050774
DSTP0212487272118533736875TP0203-TP024027
DSTP0292643233407287176406TP0253-TP032360
DSTP0463497344026785052945TP0329-TP037878
DSTP055104730611202416172936TP0966-TP102658
DSTP07697495310477906872838TP0898-TP0965n.t.
DSTP08511202429597199113742TP1028-TP008595
DSTP0946479005794855868416TP0538-TP059511
DSTP1093793112825708996742TP0267-TP035529
DSTP1476223965330367589361TP0499-TP057352
DSTP1559269381047305110120368TP0855-TP096498
DSTP173891478787554103103925TP0721-TP082397
DSTP1986847787546345969857TP0628-TP068651
DSTP2019434098865724756838TP0818-TP086442
DSTP2168134347426366970799TP0679-TP074744
DSTP2741341732118528777680TP0117-TP0203n.t.
DSTP2883831074648897681783TP0362-TP043762
DSTP3346943126479003746413TP0597-TP0633n.t.

[i] Number of ORFs amplified from each individual clone used as a template; n.t., not tested.

[ii] Position on T. pallidum chromosome based on published coordinates (Fraser et al. 1998).