Distribution of Binding-Site Matches in the eve2 Region by PWM Score
| PWM Score | Bicoid | Krüppel | Hunchback | Giant | ||||
| Exp. | Obs. | Exp. | Obs. | Exp. | Obs. | Exp. | Obs. | |
| >6 | 0 | 3 | 0 | 3 | 0–1 | 3 | 0 | 5 |
| 4–6 | 2–4 | 10 | 1–3 | 6 | 4–6 | 10 | 2–4 | 6 |
| 2–4 | 10–14 | 14 | 13–19 | 29 | 8–13 | 12 | 13–20 | 15 |
| 0–2 | 35–53 | 36 | 48–73 | 62 | 23–35 | 32 | 40–59 | 53 |
[i] The expected number of sites after random shuffling of base pairs in the eve2 sequence was evaluated for each score zone. Most of the high scoring matches (>6) found in eve stripe 2 represent experimentally verified sites. The number of shadow binding sites in the second positional weight matrix (PWM) score zone (4–6) exceeds the expected number for random sequence by at least a factor of 2.