RLGS profiles using AscI–EcoRV–HinfI restriction enzyme combination. (A) RLGS profile of normal lung DNA. (B) RLGS profile using restriction trapper purifiedAscI–EcoRV fragments derived from peripheral blood lymphocyte DNAs. (C) RLGS mixing gel generated with normal lung DNA as the genomic background and clones from plate 3 pool in the A-RV1 library. (D) Section 4C of the AscI Master RLGS profile, showing the numbers assigned to each AscI fragment.