Plasmids used in this study. In plasmid pCRG3, the 1.95-KbCYH2 gene, recombinationally cloned into the SmaI site of pRS316, is depicted as the promoter, open reading frame (ORF), and terminator that are drawn to scale. The ORF consists of two exons (shaded) separated by a 510-bp intron. Reference restriction enzyme sites from the pRS316 polylinker are indicated. In plasmid pCRG16, the high-copy-number colE1 ori, Amp^R “stuffer fragment” is liberated from the BAC recombination vector by digestion withSrfI.