Figure 2.
Single-stranded 80-nucleotide linkers support fragment subcloning. Single-strand linkers are depicted 5′ to 3′, with arrowheads at their 3′ ends. Plasmid repair was scored as Ura+ colonies, and fragment subcloning as the % CmR plasmids.
Single-stranded 80-nucleotide linkers support fragment subcloning. Single-strand linkers are depicted 5′ to 3′, with arrowheads at their 3′ ends. Plasmid repair was scored as Ura+ colonies, and fragment subcloning as the % CmR plasmids.