Self-assembling 80-bp yeast recombination linkers. (A) Complementary 80-nucleotide oligonucleotides overlap the vector and fragment by equal, 40-bp segments. (B) Using either of the two common intact-yeast-cell transformation methods, 80-bp linkers support efficient plasmid repair as monitored by the recovery of Ura⁺colonies. Direct measurements of fragment subcloning, expressed as a percent of Amp^R plasmids that had acquired Cm^R, revealed that the majority of linker-repaired plasmids harbor the targeted insert DNA.