Reactions Catalyzed by Sequence-Related Enzymes
| Gene | EC | Reaction | Mechanism |
| entE | 6.3.2.- | ATP + 2,3-dihydroxybenzoate ⇒ pyrophosphate + 2,3-Dihydroxybenzoyl-AMP | Adenylation |
| entF | — | ATP + L-serine → pyrophosphate + L-Seryl-AMP | Adenylation |
| purK | 4.1.1.21 | CO2+ H2O + AIR ⇔ ADP + phosphate + AICAR | 1 carbon incorporation |
| purT | 2.1.2.- | HCOOH + ATP + GAR ⇔ ADP + phosphate + FGAR | 1 carbon incorporation |
| guaB | 1.1.1.205 | XMP + NADH ⇔ IMP+ NAD + H2O | Redox reaction |
| guaC | 1.6.6.8 | GMP + NADPH ⇔ IMP + NADP + NH3 | Redox reaction |
| murC | 6.3.2.8 | ATP + UDP-N-acetylmuramate + L-alanine ⇔ X + phosphate + ADP | Amino acid ligation |
| murD | 6.3.2.9 | ATP + X + D-glutamate ⇔ XY + phosphate + ADP | Amino acid ligation |
| murE | 6.3.2.13 | ATP + XY + meso-diaminopimelate ⇔ XYZ + phosphate + ADP | Amino acid ligation |
| murF | 6.3.2.15 | ATP + XYZ + D-alanyl-D-alanine ⇔ XYZW + phosphate + ADP | Amino acid ligation |
| asnB | 6.3.5.4 | L-glutamine + L-aspartate + ATP + H2O ⇔ L-glutamate + L-asparagine + pyrophosphate + AMP | Amide group transfer |
| glmS | 2.6.1.16 | L-glutamine + fructose-6-phosphate ⇔ D-glucosamine-6-phosphate + L-glutamate | Amide group transfer |
| purF | 2.4.2.14 | L-glutamine + PRPP + H2O ⇔ 5-phosphoribosylamine + pyrophosphate + L-glutamate | Amide group transfer |
| menF | 5.4.99.6 | chorismate ⇒ isochorismate | Mutase |
| pabB | 4.1.3.- | chorismate + NH3 ⇔ 4-amino-4-deoxychorismate | Oxo-acid lyase |
| trpE | 4.1.3.27 | chorismate + NH3 ⇔ anthranilate + pyruvate | Oxo-acid lyase |
| gcl | 4.1.1.47 | 2 glyoxylate ⇔ CO2 + tartronate semialdehyde | Decarboxylation |
| ilvI | 4.1.3.18 | 2 pyruvate ⇔CO2 + 2-aceto-lactate (valine biosynthesis); pyruvate + 2-oxobutanoate ⇔ CO2 + 2-aceto-2-hydroxy-butyrate (isoleucine biosynthesis) | Decarboxylation |
| poxB | 1.2.2.2 | pyruvate + ferricytochrome-b1 + H2O ⇔ CO2 + acetate + ferrocytochrome-b1 | Decarboxylation |
| metB | 4.2.99.9 | L-cysteine + o-succinyl-L-homoserine ⇔ succinate +cystathionine | Carbon-oxygen lyase |
| metC | 4.4.1.8 | cystathionine + H2O ⇔ pyruvate + NH3 + homocysteine | Carbon-sulfur lyase |
| hisA | 5.3.1.16 | phosphoribosyl-formimino-AICAR-P ⇔PRFAR | Isomerization |
| hisF | 2.4.2.- | PRFAR + L-glutamine ⇔ D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate | Amidation |
[i] Sources: Gene, GenProtEC; EC (Enzyme Commission number), ExPASy–ENZYME; reaction, modified from EcoCyc; mechanism, BRENDA and EC nomenclature
[ii] In reactions catalyzed by murCDEF: X = UDP-N-acetylmuramoyl-L-alanine; XY = UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; XYZ = UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2, 6-diaminoheptanedioate; XYZW = UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2, 6-diaminoheptanedioate-D-alanyl-D-alanine.