Comparison of Crude Lysis Purification Using Either 70% Ethanol Precipitation or Solid Phase Reversible Immunobilization
| Plate Name | Pass rate ( Phred 20 >50 bp) | Average read length ( Phred 20 >50 bp) | |||
| +SPRI | −SPRI | +SPRI | −SPRI | ||
| ker0005_fw | 87.5% | 67.7% | 584 | 495 | |
| ker0006_fw | 93.8% | 76.0% | 649 | 439 | |
| ker0007_fw | 86.5% | 78.1% | 582 | 506 | |
| ker0008_fw | 95.8% | 47.9% | 516 | 281 | |
| kfn0010_fw | 95.8% | 95.8% | 614 | 536 | |
| kfn0012_fw | 78.1% | 61.5% | 491 | 492 | |
| kfn0013_fw | 94.8% | 82.3% | 614 | 518 | |
| kfn0014_fw | 96.9% | 70.8% | 597 | 429 | |
| kfn0015_fw | 92.7% | 44.8% | 537 | 347 | |
| kfn0016_fw | 88.5% | 65.6% | 632 | 421 | |
| AVERAGE | 91.0% | 69.1% | 582 | 446 | |
| Std Dev[ii] | 5.9% | 15.4% | 51.6 | 81.2 | |
[i] Plates are 96-well and contain pUC-18 plasmid with random 3–4 kb human DNA inserts from chromosomes 5 and 16. Pass rates are based on lanes producing >50 bases scoring Phred 20 or better. Read lengths are the average of the Phred 20-qualified bases in the passed lanes. Total samples, 960.
[ii] Std Dev, Standard Deviation.