[i] (A) Splice variant prediction from triplicate control experiment. Total RNA was extracted from rat heart, liver, and skeletal muscle tissues. Independent RNA labeling and chip hybridization experiments were performed as triplicate for each tissue sample. Potential splice variants were predicted from each set of triplicate data using SPLICE (SP) algorithm alone or in combination with NEIGHBORHOOD (NB) algorithm. Total number of predictions from each tissue set was calculated. (B) Splice variant prediction from three different rat tissues. To generate the data set of three different tissues, the mean CSS value of each tissue triplicate was calculated and appended into the same table. Splice variant predictions were performed using the combined data set from the three tissues.