BLAST vs. FISH for BACs with Interchromosomal Duplications: Comparison of Chromosome Assignment
| Accession no. | RPCl-11 clone | oo23 assignment (chr/contig) | Assignment consistency with FISH[i] | BLAST chr # | BLAST chr (>=90% identity >=5000 unique bases[ii]) | FISH chr # | FISH chr (Standard Metaphase Spread) | FISH chr positive by BLAST [iii] |
| AC006379 | 456n16 | 7/ctg15064 | I | 1 | 7 | 2 | 16,19 | 0 |
| AC007276 | 226o1 | 7/ctg15064 | C | 2 | 7,12 | 3 | 7,9,12 | 2 |
| AC008166 | 155j2 | 2/ctg15424 | C | 1 | 2 | 3 | 2,3,10 | 1 |
| AC009954 | 226l21 | 6/ctg16045 | l | 10 | 1,4,6,7,8,10,13,16,20,Y | 3 | 8,17,18 | 1 |
| AC010098 | 400j9 | 2/ctg14798 | C | 13 | 1,2,4,5,7,10,13,15,16, 17,21,22,U,L,Y | 10 | 1,2,7,9,10,14,15,16,17,22 | 8 |
| AC011244 | 497h16 | 5/ctg12770 | C | 4 | 5,6,20,22 | 2 | 5,6 | 2 |
| AC011881 | 17l12 | UL/ctg14414 | UL | 5 | 2,5,6,10,15,UL | 9 | 1,3,4,9,13,14,15,21,22 | 1 |
| AC012661 | 413e6 | 3/ctg14246 | C | 10 | NA,UL,Y,3,4,5,8,9,11, 15,16,17 | 18 | 1,3,4,7,8,9,10,11,12,13,14, 15,16,19,20,21,22,Y | 8 |
| AC013633 | 12p21 | 22/ctg25248 | C | 4 | 2,4,9,22,UL | 6 | 9,13,14,15,21,22 | 2 |
| AC015973 | 366c6 | 1/ctg14824 | C | 3 | 1,15,UL,Y | 3 | 1,8,Y | 2 |
| AC016745 | 480c16 | 2/ctg16335 | C | 3 | 2,6,9 | 3 | 2,8,9 | 2 |
| AC018687 | 462g22 | 4/ctg16 | C | 1 | 4 | 2 | 4,14 | 1 |
| AC018692 | 555k2 | 4/ctg25230 | C | 3 | 1,4,9 | 5 | 4,9,13,14,21 | 2 |
| AC018963 | 452l16 | 4/ctg25230 | l | 8 | 1,2,4,5,15,16,19,22,UL | 3 | 2,15,16 | 3 |
| AC020590 | 150n22 | 3/ctg17028 | C | 6 | 2,3,4,9,17,UL,Y | 12 | 3,4,9,13,14,15,16,18,20, 21,22,Y | 4 |
| AC020715 | 446k10 | 19/ctg25122 | C | 1 | 19 | 2 | 5,19 | 1 |
| AC022030 | 358b14 | 18/ctg25448 | C | 2 | 16,18 | 2 | 16,18 | 2 |
| AC022192 | 23b24 | 2/ctg100 | l | 8 | 2,5,9,10,13,15,18,21,UL | 9 | 3,4,9,13,14,15,21,22,Y | 4 |
| AC022702 | 33b1 | 4/ctg15540 | C | 8 | 1,4,6,7,9,10,13,19 | 9 | 1,3,4,7,9,10,11,16,19 | 6 |
| AC023099 | 264m14 | 16/ctg16860 | C | 8 | 2,5,10,15,16,19,22,UL,Y | 3 | 2,15,16 | 3 |
| AC024117 | 327i12 | 10/ctg13284 | C | 1 | 10 | 2 | 2,10 | 1 |
| AC024119 | 542j23 | 1/ctg17190 | C | 2 | 1,2 | 3 | 1,3,19 | 1 |
| AC024345 | 384k6 | 4/ctg15540 | C | 9 | 1,4,6,7,8,10,13,20,Y | 15 | 1,2,3,4,5,6,7,8,9,10,11,16, 19,20,Y | 8 |
| AC024410 | 125l9 | 5/ctg12474 | l | 1 | 5 | 5 | 13,14,15,21,22 | 0 |
| AC025222 | 297j4 | 9/ctg25280 | l | 5 | 1,4,9,12,15 | 2 | 1,11 | 1 |
| AC026205 | 61i9 | 3/ctg25118 | C | 1 | 3 | 2 | 3,4 | 1 |
| AC026495 | 509a17 | 15/ctg25305 | C | 6 | 1,2,4,15,16,22,UL | 10 | 1,2,9,10,13,14,15,16,17,22 | 5 |
| AL078621 | 395l14 | 2/ctg16335 | C | 11 | 2,6,7,8,9,15,16,19,21, 22,X | 16 | 1,2,3,6,7,8,9,10,11,12,15, 16,17,19,20,22 | 9 |
| AL132658 | 59j12 | 10/ctg13655 | l | 2 | 1,10 | 2 | 1,8 | 1 |
| AL133173 | 453n3 | 4/ctg3364 | l | 8 | 1,2,4,11,15,16,22,UL,X | 7 | 2,9,10,14,15,16,22 | 4 |
| AL133216 | 291l22 | 10/ctg13655 | C | 11 | 1,4,6,7,8,9,10,13,19, 20,Y | 13 | 1,3,4,5,6,7,9,10,11,16,19, 20,Y | 9 |
| AL137070 | 251o17 | 2/ctg14048 | C | 6 | 2,9,13,14,18,21 | 2 | 2,9 | 2 |
| AL138715 | 77p19 | 13/ctg14226 | C | 10 | 2,5,7,10,13,15,16,17,21, 22,UL | 7 | 2,13,14,15,18,21,22 | 5 |
| AL138998 | 119m6 | 4/ctg25230 | C | 1 | 4 | 4 | 4,13,14,21 | 1 |
| AL158811 | 139o21 | 22/ctg13153 | C | 7 | 1,2,5,7,13,21,22,UL | 8 | 1,2,7,13,14,15,21,22 | 6 |
| AL161418 | 149i22 | 22/ctg13153 | C | 7 | 1,2,5,7,13,21,22,UL | 5 | 13,14,15,21,22 | 3 |
| AL354817 | 85c8 | UL/ctg15409 | UL | 12 | 2,3,4,5,7,8,9,10,13,18, 19,21,UL | 12 | 1,2,3,4,9,13,14,15,20,21, 22,Y | 6 |
| Total | 37 | 201 | 224 | 118 |
[i] l, 8/37 Inconsistent assignment with FISH chromosomes; C, 27/37 Consistent; UL, 2/37 no chromosome assignment.
[ii] Number of bases calculated within 400 kb genomic segments.
[iii] The number of FISH chromosomes with a positive chromosome byBLAST.