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Table 4.

Error Statistics Derived from Performance Testing

MB_prim Total base calls aligned: 2,404,898
`Phred` `LifeTrace`	Correct	Subst	Insert	Del	Total `LifeTrace`
Correct	2,346,881	12,192	43,884	8,508
Subst	10,727	14,069	0	2,232	27,028
Insert	21,300	0	6,072	0	27,372
Del	4,836	1,179	0	6,072	12,087
Total`Phred`		27,440	49,956	16,812
Summary:
Both correct: 97.6% of all aligned true-sequence bases
Total `LifeTrace` errors: 64,689 = 70% of `Phred` errors, Total `Phred`errors: 92,410
Total InDels`LifeTrace`: 37,661 = 57.9% of `Phred`InDels, Total `Phred`: 64,970
Mean`BLAST` hit length to true consensus sequence,`LifeTrace`: 517.5, `Phred`: 493.9

MB_term Total base calls aligned: 2,748,823
`Phred` `LifeTrace`	Correct	Subst	Insert	Del	Total `LifeTrace`
Correct	2,691,854	11,020	33,532	8,049
Subst	10,770	15,215	0	1,434	27,419
Insert	11,573	0	3,609	0	15,182
Del	6,714	1,477	0	2,290	10,481
Total`Phred`		27,712	37,141	11,773
Summary:
Both correct: 97.9% of all aligned true-sequence bases
Total `LifeTrace` errors: 53,082 = 69.2% of `Phred` errors, Total `Phred`errors: 76,626
Total InDels`LifeTrace`: 25,663 = 52.3% of `Phred`InDels, Total `Phred`: 48,914
Mean`BLAST` hit length to true consensus sequence,`LifeTrace`: 532.3, `Phred`: 517.5

337 Total base calls aligned: 666,489
`Phred` `LifeTrace`	Correct	Subst	Insert	Del	Total `LifeTrace`
Correct	644,389	5,612	2,974	1,843
Subst	4,414	6,865	0	721	12,000
Insert	4,424	0	651	0	5,075
Del	1,671	317	0	657	2,645
Total`Phred`		12,794	3,625	3,221
Summary
Both correct: 96.7% of all aligned true-sequence bases
Total `LIfeTrace` errors: 19,720 = 100.4% of `Phred` errors, Total `Phred`errors: 19,640
Total InDels`LifeTrace`: 7,720 = 113.2% of `Phred`InDels, Total `Phred`: 6,846
Mean`BLAST` hit length to true consensus sequence,`LifeTrace`: 582.6, `Phred`: 594.2

3700 Total base calls aligned: 2,659,195
`Phred` `LifeTrace`	Correct	Subst	Insert	Del	Total `LifeTrace`
Correct	2,519,021	31,671	23,497	17,676
Subst	17,493	20,863	0	2,698	41,054
Insert	11,930	0	1,482	0	13,412
Del	34,113	5,257	0	10,403	49,773
Total`Phred`		73,397	24,979	30,777
Summary:
Both correct: 94.7% of all aligned true-sequence bases
Total `LifeTrace` errors: 104,239 = 91.8% of `Phred` errors, Total `Phred`errors: 113,547
Total InDels`LifeTrace`: 53,185 = 113.5% of `Phred`InDels, Total `Phred`: 55,756
Mean`BLAST` hit length to true consensus sequence,`LifeTrace`: 662.5, `Phred`: 705.8

[i] Break down of error statistics derived from performance testing using Method 2 (see Methods section) applied to both the MegaBACE dye-primer and dye-terminator set. Table lists all possible error combinations. For example, for the set MB_prim there were 12,192 correct calls made by LifeTrace where Phredhad a substitution error at the same position compared to 10,727 wherePhred was correct and LifeTrace had a substitution error and 14,069 cases where both basecallers had a substitution error. Mean BLAST hit length refers to the length of the high scoring sequence alignment between the called sequence and the finished, true consensus sequence. CalledNs are counted as bases and contributed to substitution and insertion errors.