Characterization of theNICE Genes
| YAC probe | Gene | Size of mRNA[i] | BLASTN (DNA): acc. no. (size), identities | BLASTP (protein): acc. no. (size), identities | Putative ORF | Putative protein domains | Positive YACs |
| 986_e_10 | NICE-1 | 694 bp | EST AI096376 (482bp), 99% | Skin-specific protein, 2589188 (110aa), 36% | 99 aa | Metallothionein, prokaryotic membrane lipoprotein lipid attachment site | 907_e_6, 874_d_5, 986_e_10, 890_e_4 |
| NICE-2 | 5500 bp | — | — | —[ii] | — | 955_e_11, 692_c_1, 100_f_3, 950_e_2 (#1, #2, #4–#11) | |
| 1710 bp | 253 aa | ||||||
| HSPC012 mRNA, | HSPC012 | N-4 cytosine-specific | 950_e_2 (#2, #4, #7, #9), | ||||
| NICE-3 | 1608 bp | AF077036 (1636bp), 99% | 4689120 (219aa), 100% | 219 aa | DNA methylases signature | 713_h_12, 951_f_6 | |
| 1554 bp | 201 aa | ||||||
| 950_e_2#9 | Bipartite nuclear localization signal, | ||||||
| 3976 bp | cDNA KIAA0144, | Hypothetical protein of | 1167 aa | ubiquitin-associated domain, | 950_e_2 (#2–#4, #7, #9), | ||
| NICE-4 | D63478 (3411bp), 99% | KIAA0144, | prokaryotic membrane lipoprotein | 713_h_12, 951_f_6, 643_h_5 | |||
| 3908 bp | 2495711 (983aa), 100% | 1068 aa | lipid attachment site (1167 aa protein only)[iii] | ||||
| Hypothetical protein of | Biotin-requiring enzymes attachment | ||||||
| NICE-5 | 2000 bp | EST AA769605(701bp), | Drosophila melanogaster, | >322aa[iv] | site, glutamine synthetase class-I | 950_e_2 (#1–#10), | |
| 99% | 2827495 (394aa), 52% | adenylation site | 951_f_6, 954_a_11 |
[i] Size as determined by Northern hybridization and confirmed by cDNA sequencing; sizes of NICE-2 andNICE-4 were only determined by Northern hybridization, because all cDNA inserts sequenced were incomplete.
[ii] No significant ORF was detected, implying that the available sequence represents the 3′ untranslated region (UTR) of the gene.
[iii] Because of space limitations, only the results of the PROSITE pattern search are indicated.
[iv] The available sequence is likely to lack the 5′ end of the ORF.